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Description
SIRP-α/CD172a mFc Chimera Protein, HumanProduct Specification Species Human Synonyms BIT; Brain Ig like molecule with tyrosine based activation motifs, CD172 antigen like family member A; Macrophage fusion receptor; MFR; MYD1; P84; protein tyrosine phosphatase; non receptor type substrate 1; PTPNS1; SHP substrate 1; SHPS1; SHPS1CD172A; Signal regulatory protein alpha 2; SIRP alpha Accession P78324 1 Amino Acid Sequence Glu31 Arg370, with C terminal mFc Expression System HEK293 Molecular
Product Specification
| Species | Human |
| Synonyms | BIT; Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A; Macrophage fusion receptor; MFR; MYD1; P84; protein tyrosine phosphatase; non-receptor type substrate 1; PTPNS1; SHP substrate 1; SHPS1; SHPS1CD172A; Signal-regulatory protein alpha-2; SIRP alpha |
| Accession | P78324-1 |
| Amino Acid Sequence | Glu31-Arg370, with C-terminal mFc |
| Expression System | HEK293 |
| Molecular Weight | 77-90kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | Mouse Fc Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4. |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | Barclay, A.N. & M.H. Brown (2006) Nat. Rev. Immunol. 6:457. 2. vanBeek, E.M. et al. (2005) J. Immunol. 175:7781. 3.Liu, Y. et al. (2005) J. Biol. Chem. 280:36132. 4.Kharitonenkov, A. et al. (1997) Nature 386:181. |
Background
Signal regulatory protein alpha (SIRP alpha, designated CD172a), also called SHPS-1 (SHP substrate 1) and previously, MyD-1 (Myeloid/Dendritic-1), is a monomeric ~90 kDa type I transmembrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin superfamily. SIRPs are paired receptors, with similar extracellular domains but differing C-termini and functions. The 503 amino acid (aa) human SIRP alpha contains a 342 aa extracellular domain (ECD), with one V-type, and two C1 type Ig domains, and three potential N glycosylation sites. It has a 110 aa cytoplasmic sequence with ITIM motifs that recruit tyrosine phosphatases SHP-1 and SHP-2 when phosphorylated. SIRP alpha is expressed mainly on myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells. It is also found on neurons, smooth muscle and endothelial cells. SIRP alpha shows adhesion to the ubiquitous CD47/IAP (integrin associated protein), while SIRP gamma binds moreweakly and SIRP alpha 1 does not bind at all. Mouse and human SIRP alpha-CD47 binding only cross-reacts for specific polymorphisms and influences engraftment of xenotransplanted stem cells. SIRP alpha engagement generally produces a negative regulatory signal. Low SIRP alpha recognition of CD47, which occurs on aged erythrocytes or platelets or xenogenic cells, promotes clearance of CD47low cells from circulation. SIRP alpha recognition of surfactants SP-A and SP-D in the lung can inhibit alveolar macrophage cytokine production.
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